Fig. 7.
Talampanel prevents in vivo monocyte-induced photoreceptor apoptosis. Two- to three-month-old Cx3cr1GFP/GFP mice were exposed for 4 days to constant light. On the fifth day, mice were injected IP every 2 h with Vehicle (CTL) or Talampanel in 0.5% tween-20. After 10 h, mice were sacrificed and Choiroid/RPE and retina were dissected. a Representative microphotographs of choroidal flatmounts of CTL- and Talampanel-treated mice immunostained with IBA1. b Quantification of the number of subretinal IBA1-positive cells in CTL- and Talampanel-treated mice. c Representative microphotographs of retinal flatmount of CTL- and Talampanel-treated mice stained by TUNEL. d Quantification of the number of TUNEL-positive cells in CTL- and Talampanel-treated mice. Scale bar 100 μm. (n = 8/group, Mann–Whitney test, *p = 0.021)