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. 2020 Jan 3;20:3. doi: 10.1186/s12935-019-1078-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — UBQLN4 regulates proliferation and invasion of HCC cells through wnt-β-catenin pathway. a The Gene Set Variation Analysis (GSVA) plot indicated a significant correlation between UBQLN4 and wnt-β-catenin pathway. b–e the Gene Set Enrichment Analysis (GSEA) showed the extensive positive correlation between UBQLN4 expression and Wnt pathway-related genes and stemness signature genes. f Dual luciferase assay demonstrating the effect on TOP/FOP reporter activity in Hep-G2 and SMMC-7721 cells transfected with shUBQLN4 and negative control. Results were normalized to a Renilla transfection control. g–i Expression change of β-catenin, CyclinD1, Axin2, Wnt5a and C-my in HCC cells and xenograft mouse models bearing tumors under condition of UBQLN4 suppression. j Showed UBQLN4 expression under different condition of negative control, shUBQLN4, vector plasmid, β-catenin plasmid or β-catenin plasmid and shUBQLN4. k Cell viability of SMMC-7721 cells under treatment of negative control, shUBQLN4, vector plasmid, β-catenin plasmid or β-catenin plasmid and shUBQLN4 in 24 h, 48 h and 72 h. *p < 0.05, **p < 0.01