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. 2020 Jan 3;24:2. doi: 10.1186/s40824-019-0180-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Immunocytochemistry analysis of osteogenitor markers (collagen I, osteopontin and osteocalcin) for human osteoblast cells cultured in: (a, b, c) Osteogenic medium (d, e, f) Basal medium after 14 and 30 days; e: Offset.50.50; f: Gradient; g: Tissue culture plate (TCP). Merge: Hoechst staining of the nucleus (blue), staining of the antibodies (green fluorescence); (Scale bar = 100 μm)