. 2020 Jan 3;11:14. doi: 10.1186/s13287-019-1533-1

Table 1.

Media formulation

Media	Basal media	Supplements
MET induction medium (M1)	MesenPRO-RS™ (ThermoFisher)	Vaporic acid (VPA, 500 μM; Sigma-Aldrich), CHIR99021 (3 μM; Stemgent), E-616452 (RepSox, 1 μM; Millipore), Tranylcypromine (5 μM; Tocris), A-83-01 (500 nM; Tocris) All-trans retinoic acid (atRA, 10 μM; Sigma-Aldrich) Serum (2%, supplied from MesenPro kit) Antibiotics-antimycotic (Invitrogen)
MET induction medium (M2)	MesenPRO-RS™	Same as M1 with reduced VPA (50 μM) and CHIR99021 (300 nM)
M2/CNT-50 medium		M2 and CNT-50 medium (CELLnTEC, Bern, Switzerland) (1:1 v/v)

Media

Basal media

Supplements

MET induction medium (M1)

MesenPRO-RS™

(ThermoFisher)

Vaporic acid (VPA, 500 μM; Sigma-Aldrich),

CHIR99021 (3 μM; Stemgent),

E-616452 (RepSox, 1 μM; Millipore), Tranylcypromine (5 μM; Tocris),

A-83-01 (500 nM; Tocris)

All-trans retinoic acid (atRA, 10 μM; Sigma-Aldrich)

Serum (2%, supplied from MesenPro kit)

Antibiotics-antimycotic (Invitrogen)

MET induction medium (M2)

MesenPRO-RS™

Same as M1 with reduced VPA (50 μM) and CHIR99021 (300 nM)

M2/CNT-50 medium

M2 and CNT-50 medium (CELLnTEC, Bern, Switzerland) (1:1 v/v)