Fig. 5.

Increased microglial activation 24 h after experimental meningitis induction. Immunofluorescence analysis for IBA-1-positive cells in experimental meningitis rats and control rats. a, b Representative microscopic field images (magnification, × 400) immunostained with IBA-1 antibodies in the PFC for 24 h. c, d Representative microscopic field images (magnification, × 200) immunostained with IBA-1 antibodies in the hippocampus for 24 h. The number of IBA-1-positive cells/1 × 10⁻² mm² in the e PFC and f hippocampus after 24 h. The results are expressed as the mean ± SEM for n = 4–5 rats. **P < 0.01 compared to controls