Fig. 1.
a Treatment setup: Tumor inoculation at day 0. Whole-cell vaccine subcutaneous immunization with 2 × 106 irradiated (40 Gy) tumor mouse glioma cells (GL261, or KR158 cells) at day 5, 19, and 33. CED-TMZ between days 7–9 administered via micro-osmotic pump/brain infusion kit (total dose of 180 μg) or via single bolus injection in 5 μl. Non-treated mice or micro-osmotic pump filled with NaCl 0.9% were use as controls. Survival was monitored for 100 days. Kaplan Meyer survival curves display the therapeutic effect of CED-TMZ and/or immunotherapy in C57BL/6 mice bearing orthotopically syngeneic gliomas. Two independent experiments per cell line were pooled. Log-rank test analysis showed significant prolonged median survival relative to respective non-treatment groups in (b) GL261 (n = 80): CED-TMZ + GL261 vs. non-treated (****p < 0.0001). CED-TMZ vs. non-treated (****p < 0.0001). GL261 vs. non-treated (***p = 0.0006). CED-TMZ + GL261 vs. CED-TMZ (**p = 0.0038). CED-TMZ + GL261 vs. GL261 (****p < 0.0001); and (c) KR158 (n = 64): CED-TMZ + KR158 vs. non-treated (****p < 0.0001). CED-TMZ vs. non-treated (*p = 0.0196). KR158 vs. non-treated (****p < 0.0001). CED-TMZ + KR158 vs. CED-TMZ (*p = 0.04169). KR158 vs. CED-TMZ (*p = 0.0142)