Fig. 6.

HULC enhances pRB and inhibit P21 WAF1/CIP1 in live cancer stem cells. a Co-IP with anti-PKM2 followed by Western blotting with anti-LC3. b Western blotting with anti-PKM2 in three hLCSC lines. c Western blotting with anti-CyclinD1 in three hLCSC lines. d Co-IP with anti-CyclinD1 followed by Western blotting with anti-CDK4. IgG IP served as negative control. Western blotting with anti-CDK4 served as INPUT. e Western blotting with anti-pRB and anti-P21WAF1/Cip1. β-actin served as internal control