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. 2020 Jan 3;11:8. doi: 10.1186/s13287-019-1528-y

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — CyclinD1 is required for HULC oncogenic action. a RT-PCR for HULC and Western blotting with anti-CyclinD1. β-actin served as internal control. b Cell growth assay using CCK8. c Colony formation assay. d The picture of xenografted tumors from mouse. e The wet weight of xenografted tumors from mouse. f The appearance time of xenograft tumor. g PCNA staining (DAB staining, original magnification× 100). h PCNA positive rate analysis