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. 2020 Jan 3;11:13. doi: 10.1186/s13287-019-1534-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — NTA study of MSC-derived EVs at several ages. a Representative results from the NTA assay of old and young MSC-derived EVs. b Electron micrographs of MSC-derived EVs from the old and young groups of rats (scale bar = 100 nm). c The number of particles per cell at different age groups by NTA assay. d Concentration of protein per particle at different age groups as determined by NTA assay. e Mean size of particles, expressed in nanometres, for different age groups as determined by NTA assay. *P < 0.05 compared with the former group was considered statistically significant using Mann-Whitney U and Kruskal-Wallis tests. f Ponceau S staining for protein content for 20 μg MSC-derived EVs and their immunoblot staining for exosome markers CD63, TSG101 and Calnexin