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. 2020 Jan 3;11:13. doi: 10.1186/s13287-019-1534-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Internalisation of MSC-derived EVs as observed by fluorescence microscopy. Images from fluorescence microscopy (magnification, × 40) of the nuclei of MSCs stained with 4′,6-diamidino-2-phenylindole (DAPI) and MSC-derived EVs stained with DiI. a Old MSCs cultured with young MSC-derived EVs at 2, 3 and 6 days. b Young MSCs cultured with old MSC-derived EVs at 2, 3 and 6 days. c Control of young MSCs and old MSCs without added EVs. One representative experiment is shown. oMSC, MSCs from the old group; yMSC, MSCs from the young group; oEV, MSC-derived EVs from the old group; yEV, MSC-derived EVs from the young group