Fig. 4.

Study of the self-renewal potential in MSCs. a Histograms represent Nanog and Oct4 gene expression normalised with HPRT, a housekeeping gene, in old MSCs with or without young MSC-derived EVs (upper). Histograms represent Nanog and Oct4 gene expression normalised with HPRT, a housekeeping gene, in young MSCs with or without old MSC-derived EVs (bottom). b The histograms represent the gene expression of vinculin normalised by HPRT, a housekeeping gene, in old MSCs with or without young MSC-derived EVs (upper). The histograms represent the gene expression of vinculin gene expression normalised by HPRT, a housekeeping gene, in young MSCs with or without old MSC-derived EVs (bottom). *P < 0.05 compared with the former group was considered statistically significant using Mann-Whitney U and Kruskal-Wallis tests. Control, MSCs cultured with growth medium without EVs; oMSC, MSCs from the old group; yMSC, MSCs from the young group; oEV, MSC-derived EVs from the old group; yEV, MSC-derived EVs from the young group