Fig. 6.
The culture of MSC-laden HyStem-C constructs under cLIUS stimulation. MSCs were encapsulated at a density of 5 × 106 cells/ml of HyStem-C hydrogel and grown in DMEM medium supplemented with 10% FBS, 100 nM dexamethasone, and 50 μg/ml l-ascorbic acid for 6 weeks under cLIUS at 14 kPa (5 MHz, 2.5 Vpp), 20 min/application, and 4 applications/day (n = 3). Non-cLIUS-stimulated 3D constructs served as controls (n = 3). Representative images of 4-μm sections of the constructs stained immunohistochemically for collagen II and chondroitin sulfate is shown. Scale bar represents 100 μm