Fig. 2.

Effect of Gm14461 expression on the expression of CGRP, P2X3 receptor, and P2X7 receptor in primary TGNs. a The primary mouse TGNs were isolated from C57BL/6 J mice and treated with TNF-α (10 ng/mL), IL-1β (25 ng/mL) and IL-6 (25 ng/mL) for 24 h. Gm14461 expression in TGNs was examined by qRT-PCR. ^*P < 0.05 vs. Control. b Western blot analysis of protein levels of CGRP, P2X3 receptor, and P2X7 receptor in primary TGNs transfected with scramble siRNA, si-Gm14461, empty vector, and pcDNA3.1-Gm14461 in the stimulation of TNF-α (10 ng/mL) for 24 h. c The qualifications of western blots in (b). Data are presented as mean ± SD. N = 3. ^*P < 0.05 vs. Control; ^#P < 0.05 vs. TNF-α + Scramble siRNA;^$P < 0.05 vs. TNF-α + Vector