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qRT-PCR analysis of DEGs in P. koraiensis under cold stress. Transcript levels of 12 randomly selected DEGs, including 6 upregulated (a) and 6 downregulated (b). The Y-axis on the left shows the relative gene expression levels (2-ΔΔCt) analyzed by qRT-PCR (blue lines), while Y-axis on the right shows corresponding expression data of RNA-seq (red dotted lines). The X-axis represents the time (hours) of − 20 °C treatment
