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. 2019 Dec 10;111(1):175–185. doi: 10.1111/cas.14233

Figure 2.

Figure 2

Neurogenic differentiation factor 1 (NeuroD1) regulates the expression level of cell cycle regulator p21. A, Relative luciferase activity of p21‐promoter reporter vector (p21‐luc) in NeuroD1‐silenced HCT116 cells. B and C, p21 mRNA expression level in NeuroD1‐silenced and NeuroD1‐overexpressed HCT116 cells, respectively, as examined using quantitative RT‐PCR (qPCR). D and E, p21 protein expression level in NeuroD1‐silenced and NeuroD1‐overexpressed HCT116 cells, respectively, as determined using western blotting. F, Cell number of NeuroD1, p21‐double silenced HCT116 cells at indicated time points. β‐Actin was used for qPCR normalization and as western blotting loading control. Cells transfected with shCon or pcCon were used as controls. Quantitative data were expressed as mean ± SD from three independent experiments. shND1, shRNA expression vector targeting NeuroD1; pcCon, pcDNA3.1(+); pcND1, NeuroD1 overexpression vector; *P < 0.05; **P < 0.01