Figure 3.

Effect of HULC knockdown in tumor xenografts in vivo. Xenograft tumors were established following ex vivo transfection of Panc‐1 cells with an siRNA to HULC or a control siRNA. A, Tumor volume was estimated at the indicated time points. Data are mean estimated tumor volumes from 3 separate xenografts. B, Tumors were excised at 4 weeks after implantation. Bars are means ± SEM xenograft tumor weights. Scale bar = 10 mm. C, RNA was isolated from xenograft tumors and quantitative PCR was carried out for HULC, E‐cadherin, N‐cadherin, vimentin, and Snail. D, Protein was isolated from xenograft tumors and western blotting was undertaken using Abs against E‐cadherin, N‐cadherin, vimentin, and Snail. Relative expression normalized to that of β‐actin is shown. Bars are means ± SEM of 3 independent experiments. *P < .05