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. 2019 Nov 22;111(1):127–136. doi: 10.1111/cas.14224

Figure 2.

Figure 2

Oxyfedrine (OXY) sensitizes cancer cells to the effects of glutathione (GSH) depletion by sulfasalazine (SSZ) and buthionine sulfoximine (BSO). A, Intracellular content of GSH in HCT116 and HSC‐4 cells incubated for 48 h in the absence or presence of SSZ (400 µmol/L), BSO (100 µmol/L), or OXY (50 µmol/L) as indicated. Data are expressed relative to the corresponding value for control cells and are means ± SD from three independent experiments. *P < .05, **P < .01 (Student's t test). B, HCT116 and HSC‐4 cells were cultured for 48 h as in (A) and were then assayed for cell viability. Data are means ± SD from three independent experiments. **P < .01 (Student's t test). C, HCT116 and HSC‐4 cells cultured as in (A) for 24 h were subjected to immunofluorescence analysis of 4‐HNE (green). Nuclei were also stained with DAPI (blue). Scale bars, 100 µm. D, HCT116 and HSC4 cells cultured as in (A) for 48 h were assayed for reactive oxygen species by flow cytometric analysis after loading with chloromethyl‐dihydrodichlorofluorescein diacetate (CM‐H2DCF‐DA; Life Technologies)