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. 2019 Dec 31;12:11623–11635. doi: 10.2147/OTT.S218308

Figure 2.

Figure 2

Knockdown of EGOT inhibited the malignant phenotypes of HCC cell line Huh7. (A) qRT-PCR was used to verify the efficiency of knockdown of EGOT by RNA interfering. (B) CCK-8 method was used to detect the proliferation of Huh7 cells after EGOT knockdown. (C) Colony formation assay was conducted to evaluate the ability of colony formation of Huh7 cells after EGOT knockdown. (D) Wound-healing assay was used to examine the motility of Huh7 cells after EGOT knockdown. (E, F) Transwell assays were used to detect the migration and invasion of Huh7 cells after EGOT knockdown, respectively. (G) The apoptosis of Huh7 cells after EGOT knockdown was analyzed by flow cytometry. *P<0.05, **P<0.01, ***P<0.001.