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. 2019 Dec 31;12:11623–11635. doi: 10.2147/OTT.S218308

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© 2019 Wu et al.

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miR-33a-5p reverse the effects of EGOT on the expression of HMGA2 and malignant phenotypes of HCC cells. (A–C) qRT-PCR was used to detect the expression level of EGOT, miR-33a-5p and HMGA2 after transfection. (D) CCK-8 method was used to detect the proliferation of Huh7. (E) Colony formation assay was conducted to evaluate the ability of colony formation of Huh7 cells. (F) Wound-healing assay was used to examine the motility of Huh7 cells. (G, H) Transwell assays were used to detect the migration and invasion of Huh7 cells, respectively. (I) The apoptosis of Huh7 cells was analyzed by flow cytometry. *P<0.05, **P<0.01, ***P<0.001.