TABLE 1.

Intensity Decay Analysis of [Ru(bpy)₂(phen-mi)]²⁺ and When Conjugated to HSA, IgG, and β-Gal^a

Protein	τ1 (ns)	αi	fi	ns	${\chi }_{\text{R}}^2$b
Free/Ar	364	0.365	0.195
	860	0.635	0.805	763	2.9 (29.4)c
Free/air	216	0.259	0.126
	521	0.741	0.874	483	2.1 (17.2)
HSA/Argon	62	0.527	0.066
	650	0.277	0.360
	1458	0.195	0.574	1076	1.0 (19; 119)
HSA/air	72	0.481	0.079
	568	0.357	0.458
	1261	0.162	.463	850	1.6 (15; 852)
β-Gal/Ar	66	0.442	0.052
	679	0.380	0.460
	1533	0.178	0.488	1064	1.6 (16; 582)
β-Gal/air	56	0.448	0.063
	512	0.429	0.552
	1248	0.123	0.385	767	3.9 (19; 499)
IgG/Ar	92	0.308	0.038
	783	0.403	0.422
	1403	0.289	0.542	1094	1.2 (4; 200)
IgG/air	28	0.461	0.039
	415	0.253	0.319
	737	0.286	0.642	607	1.4 (4; 256)

^aMeasurements were performed at 23°C, in the presence (air) and absence (Ar, argon) of dissolved oxygen from the air.

^bFor calculation of ${\chi }_{\text{R}}^2$, we used δϕ = 0.2 and δm = 0.007.

^cValues in parenthesis show ${\chi }_{\text{R}}^2$ for two- and one-component fits.