. Author manuscript; available in PMC: 2020 Jan 4.

Published in final edited form as: Anal Biochem. 1997 Sep 5;251(2):241–245. doi: 10.1006/abio.1997.2253

TABLE 2.

Anisotropy Decay Analysis of [Ru(bpy)₂(phen-mi)]²⁺ Conjugated to Proteins, HSA, IgG, and β-Gal^a

Protein	ϕ_i (ns)	r_Oi	Σr_Oi	$χ_{R}^{2}$
HSA	42.6	0.158	0.158	5.3^b
β-Gal	26	0.091
	498	0.026	0.117	4.4
IgG	28	0.034	0.014	3.0
	17	—	〈0.15〉	4.1

See Table 1 for additional details.

The value of χ was calculated using δϕ = 0.2 and δm Å 0.007.