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. 2019 Dec 31;12:11667–11677. doi: 10.2147/OTT.S231125

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© 2019 Liu et al.

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Mcl-1 mediates the effects of miR-375 on MG63 cell growth, migration and DNA damage during Cis treatment. MG63 cells, which were transfected with siMcl-1-1 or Mcl-1 before transfection with miR-375-mimics, were treated with Cis (10 μM). (A, B) RT-PCR showing the rescue of the Mcl-1-induced downregulation of miR-375 by siMcl-1-1 in MG63 cells and the inhibition of miR-375 by Mcl-1 in MG63 cells. Data are shown as the mean ± s.e.m., n = 5, *p< 0.05, **p< 0.01, ANOVA. (C) Growth assay showing that Mcl-1 overexpression partially rescues the proliferative effects of miR-375 inhibition in MG63 cells, n=3, Student’s t-test, *p<0.05. (D) Representative images of a wound healing assay 0 and 24 h after scratching. (E) Percentage of scratch closure is shown as the mean ± s.e.m., n = 5, *p< 0.05, ANOVA. (F) Representative images of the comet assay. (G) Percentage of DNA in the tail is shown as the mean ± s.e.m., n = 5, *p< 0.05, ANOVA.