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. Author manuscript; available in PMC: 2020 Jul 3.
Published in final edited form as: Science. 2019 Nov 14;367(6473):100–104. doi: 10.1126/science.aaz4352

Fig. 2. Mechanism of ribosome-nascent chain engagement by TTC5.

Fig. 2

(A) Overview of the cryo-EM derived structure of the complex between TTC5 and a ribosome containing the first 64 amino acids of β-tubulin. (B) Close-up view of the TTC5 interaction with 28S rRNA. Three conserved lysine residues in TTC5 within electrostatic interaction distance of the rRNA backbone are indicated. (C) The surface of TTC5 that interacts with ribosomal protein uL24 is indicated in orange. The 28S-interacting residues from panel B are shown in yellow. The N-terminal 8 amino acids of the β-tubulin nascent chain is shown in red within its binding groove of TTC5. (D) Close-up view of the N-terminal 8 amino acids of nascent β-tubulin (MREIVHIQ) within TTC5. Yellow spheres denote C-β atoms for the indicated side chains (not modelled). R147 is within salt-bridge distance of E3, and D225 and E259 are within salt-bridge distance of R2. F194 on the ‘floor’ of the binding groove, shown in Fig. 3A to crosslink with nascent β-tubulin, is indicated.