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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Nanomedicine. 2019 Oct 25;23:102094. doi: 10.1016/j.nano.2019.102094

Figure 2.

Figure 2.

Intracellular uptake of PgP/DS RNA polyplexes assessed in MDA-MB-231 human breast cancer cells. (A) Visualization of intracellular uptake of polyplexes by confocal microscope; (B) Co-localization of polyplexes with early endosomal marker Rab5 confirms an endocytic pathway of internalization; (C) Flow cytometry measuring the effect of temperature on intracellular uptake of polyplexes (**** denotes p < 0.0005); (D) Fluorescent microscopy and bright-field overlay of MDA-MB-231 human breast cancer cells transfected with either PgP/DS RNA-Al488 or PgP/DNA-Al488 at either 4 °C or 37 °C, 6 hours after transfection.