(A) AFM images of RhoA NANPs and (B,C) specific gene silencing with PgP/NANP polyplexes targeting RhoA in rat neuroblastoma cells (B35). In all experiments, cubes (PgP/cubes(RhoA)), rings (PgP/rings(RhoA)), fibers (PgP/fibers(RhoA)), and individual DS RNAs(RhoA) are compared. Negative control siRNA (siNT) and DS RNA transfected with Lipofectamine 3000 (L3K) are used as controls. Intracellular uptake of fluorescently labeled PgP/Ring-Al488 by confocal microscopy (A) and RhoA gene silencing assessed by RT PCR (B).