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. 2019 Nov 28;104(1):131–144. doi: 10.1007/s00253-019-10242-1

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Correlation of gene copy numbers and cell counts. A clear separation of ice (circles) and LN (triangles) samples could be observed. The arrows indicate the gene copy numbers of the negative controls. A clear separation of ice (circles) and LN (triangles) samples could be observed. The negative control (diamonds, NC_B) with the highest cell number determined the threshold for the detection limit, illustrated by a vertical dashed line. The axes are log₁₀-scaled. The LN samples are in the range of the negative controls. The debris samples (squares) had the highest gene copy numbers and cell counts. The concentration is calculated per ml evaporated LN, thawed ice, air volume reaction tube (NC_B)