Table 1.
Antibodies and techniques used for each method of immunohistochemical staining
| Antibody | Clone | Supplier | Catalog number | Species | Platform | Retrieval | Antibody dilution and incubation | Detection chemistry |
|---|---|---|---|---|---|---|---|---|
| p53 | DO-7 | Dako | M7001 | Mouse | Bond RX | Epitope retrieval solution 1 (ER1) 30MINS | 1/1000 15MINS | Bond polymer DAB refine + Enhancer |
| GLUT-1 | NA | Roche Ventana | 760-4526 | Rabbit polyclonal | Ventana benchmark | Cell conditioning solution 1 (CC1) 8 min | pre diluted | Optiview DAB Kit |
| CD8 | C8/144B | Dako | M7103 | Mouse | Bond RX | Epitope retrieval solution 2 (ER2) 20mins | 1/50 15mins | Bond polymer DAB refine + enhancer |
| HER4B5 | 4B5 | Roche Ventana | 790-2991 | Rabbit | Ventana benchmark | Cell conditioning solution 1 (CC1) 30 min | pre diluted | Ultraview DAB IHC Kit |
PET responder defined as a reduction in standardized uptake values of > 35%
Positive circumferential resection margin defined as tumor at or within 1 mm of the circumferential resection margin
PET-positive emission tomography scan, T stage tumor stage, N stage nodal stage