Fig. 1.

Amount of p53 protein (a) and its mRNA transcripts (c) in hippocampus of 4- and 24-month-old IL-6-deficient (IL-6KO) and wild type control (WT) mice. Level of mRNA expression was defined as log2(FC), where FC stands for fold-change difference in mRNA level between indicated groups. Bars represent mean ± SEM obtained from six animals in each group. Expression of p53 protein was low and comparable in 4-month-old groups of both genotypes. In 24-month-old WT mice the amount of p53 protein increased significantly in comparison with 4-month-old WT (**p < 0.01) and 24-month-old IL-6KO (*p < 0.05) animals (Kruskal–Wallis with Dunn’s post hoc test). According to GLM the accumulation of p53 protein was both genotype- and age-dependent (p < 0.005 and p < 0.05, respectively). c There were no significant differences in the p53 mRNA levels between four groups of mice, however, according to GLM analysis the p53 mRNA level turned out to be influenced by genotype (p < 0.05). b Representative immunoblot for p53 protein is shown together with α-tubulin as a loading control. M molecular weight marker