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. 2019 Oct 9;21(1):29–43. doi: 10.1007/s10522-019-09841-2

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — a Representative microphotographs of Hoechst 33258 and TUNEL staining of hippocampal dentate gyrus form 4- and 24-month-old IL-6-deficient (IL-6KO) and wild type control (WT) mice revealed single apoptotic cells in hippocampus of aged mice of both genotypes, (magnification × 200). b (a) Fluorescent Hoechst 33258 (blue) nuclear staining, (b) TUNEL staining (green) indicating apoptotic nucleus, (c) Merged images of Hoechst 33258 (blue) and TUNEL (green) staining, (magnification × 400). White arrow indicates apoptotic cell