Table 3.
Effects of genotype and age on hippocampal mRNA transcript levels measured by qRT-PCR in 4- and 24-month-old IL-6-deficient (IL-6KO) and wild type control (WT) mice
| Parameter | Groups (mean ΔCT ± SEM), n = 12 | p values | |||||
|---|---|---|---|---|---|---|---|
| ΔCT for gene | WT | IL-6KO | Young adult (4 m) | Aged (24 m) | Genotype | Age | Genotype*age interaction |
| p53 | 4.7332 ± 0.0582 | 4.4588 ± 0.0602 | 4.6615 ± 0.0642 | 4.6254 ± 0.0605 | 0.0359 | > 0.05 | > 0.05 |
| Mdm2 | 1.5632 ± 0.0519 | 1.3695 ± 0.0408 | 1.3547 ± 0.0392 | 1.5773 ± 0.0505 | 0.0026 | 0.0006 | > 0.05 |
| Pten | − 0.4502 ± 0.0837 | − 0.7827 ± 0.0473 | − 0.7470 ± 0.0706 | − 0.4843 ± 0.0736 | 0.0008 | 0.0076 | > 0.05 |
| Tsc2 | 1.7806 ± 0.1240 | 1.2768 ± 0.0586 | 1.4002 ± 0.1118 | 1.6626 ± 0.0753 | 0.0007 | > 0.05 | > 0.05 |
| Sesn1 | 4.0593 ± 0.1033 | 3.8600 ± 0.0759 | 3.7620 ± 0.0921 | 4.1530 ± 0.1058 | > 0.05 | 0.0020 | > 0.05 |
| Dram1 | 6.9689 ± 0.1003 | 6.6295 ± 0.0717 | 6.7184 ± 0.0948 | 6.9795 ± 00793 | 0.0385 | > 0.05 | > 0.05 |
Bold p values indicate significant influence of a given factor according to General Linear Model (GLM)