Table 1.

Sensitivity of the BY4741 strain and isogenic mutant strains sod1Δ, sod2Δ, rad52Δ treated with curcumin on cells stressed factors

Strain	Growth conditions
	YPD	Congo red 100 μg/mL	MMS 0.03%	NaCl 1 M	H2O2 5 mM	SDS 0.01%	SDS 0.02%	Acetic acid 40 mM	Heat shock
BY4741	++++	++	+++	+++	+++	++++	+++	++++	++++
BY4741 + curcumin 200 µM	++++	++	+++	+++	++	++++	+++	++++	++++
BY4741 + curcumin 300 µM	++++	+++	+++	+++	++	+++	++	+++	++++
sod1Δ	++++	+	+++	+++	++	+++	+++	+++	++++
sod1 Δ + curcumin 200 µM	++++	+	+++	++	+	+++	++	++	++++
sod1 Δ + curcumin 300 µM	++++	++	+++	++	+	++	+	+	++++
sod2Δ	++++	++	+++	+++	++	+++	+++	+++	++++
sod2 Δ + curcumin 200 µM	++++	+++	+++	+++	+	+++	++	+++	++++
sod2 Δ + curcumin 300 µM	++++	+++	+++	+++	+	++	+	++	++++
rad52Δ	++++	+	−	+++	+	+++	+++	+++	++++
rad52 Δ + curcumin 200 µM	++++	++	−	+++	+	+++	++	+++	++++
rad52 Δ + curcumin 300 µM	++++	++	−	+++	+	++	+	+++	++++

Cells were cultured overnight in liquid YPD medium, counted and diluted serially to obtain suspensions at the densities 10⁷, 10⁶,10⁵ and 10⁴ cell/mL. Five microliters of each suspension was spotted at YPD solid medium containing various concentrations of curcumin and analyzed toxic factors. Plates were cultivated 2 days in 28 °C. Each “+” means the growth of one spot containing respectively: 50,000, 5000, 500, 50 cells