A) High levels of IQGAP1 correlates with poorer survival in HNC patients. Kaplan-Meier curve was used to analyze how IQGAP1 expression level correlates with head and neck cancer survival, based on TCGA RNA database. Patients with higher IQGAP1 expression (N=346) results in poorer survival probability than those with lower IQGAP1 expression (N=153). The log-rank pValue< 0.0001, two-sided. B) Head and neck cancer TMA showed significant positive correlation between IQGAP1 and PI3K signaling, represented by pS6 level. Spearman Rank correlation test was performed in the p16-negative subset of samples (N=162), resulting in a coefficient rho=0.281, indicating a weak positive correlation the two markers. The observed positive correlation is statistically significant (p=0.0003, two-sided). The TMA slide was scanned and imaged by Vectra imaging system. Images were analyzed and quantified by inForm® Tissue Finder software. Levels of expression were reported as H-Scores