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. 2019 Dec 20;2019:5390720. doi: 10.1155/2019/5390720

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Copyright © 2019 Hitomi Kuramoto et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

VEGF receptor mRNA expressions in CAPE-treated KN-3 cells in normal medium and osteogenic induction medium. KN-3 cells were stimulated with caffeic acid (10 μg/ml), CAPE (10 μg/ml), or EGCG (10 μg/ml) for 6 hours in normal medium or osteogenic induction medium. After stimulation, total RNA was isolated and mRNA expression levels of VEGFR-1 and VEGFR-2 were analyzed by real-time RT-PCR. (a) VEGFR-1 mRNA expression levels in normal medium. (b) VEGFR-1 mRNA expression levels in osteogenic induction medium. (c) VEGFR-2 mRNA expression levels in normal medium. (d) VEGFR-2 mRNA expression levels in osteogenic induction medium. Values represent the means ± SDs representative of three independent experiments, and each experiment was performed in triplicate. Asterisks indicate significant differences versus the control (without polyphenols) (^∗p < 0.05 vs. control).