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. 2019 Dec 20;2019:5390720. doi: 10.1155/2019/5390720

Figure 9.

Figure 9

VEGF-induced mineralization activity in KN-3 cells under osteogenic induction condition. KN-3 cells cultured with osteogenic induction medium containing VEGF for 10, 13, or 17 days. (a) ALP activity was determined using LabAssay ALP (Wako) and normalized by protein amount. Values represent the means ± SDs from representative of three independent experiments, and each experiment was performed in triplicate. Asterisks indicate significant differences versus the control (without VEGF) (∗p < 0.05). (b) Alizarin Red S-staining of KN-3 cells cultured with VEGF (100 ng/ml) for 10, 13, or 17 days. (c) Quantification of the Alizarin Red S-stained area was analyzed by the measurement of absorbance at 405 nm using a microplate reader after the elution with 5% formic acid. Values represent the means ± SDs from representative of three independent experiments, and each experiment was performed in triplicate. Asterisks indicate significant differences versus the control (without VEGF) (∗p < 0.05).