Table 1.

Oligonucleotide sequences of primers used for RT-PCR.

Target gene	Primer	Sequence	Size of PCR fragment (bp)	PCR cycles	Annealing temperature (°C)
DMP-1	Sense	5′-GGACGGCTCTGAGTTCGA-3′	258	36	56
	Antisense	5′-TGGGTTTCCCTGCTGTTG-3′
DSPP	Sense	5′-TCAATGGCGGGTGCTTTAGA-3′	110	40	60
	Antisense	5′-TGCTCACTGCACAACATGAAGA-3′

DMP-1, dentin matrix protein- (DMP-) 1; DSPP, dentin sialophosphoprotein.