Table 2.
Oligonucleotide sequences of primers used for quantitative real-time PCR.
| Target gene | Primer | Sequence |
|---|---|---|
| VEGFA | Sense | 5′-AAAAACGAAAGCGCAAGAAA-3′ |
| Antisense | 5′-TTTCTCCGCTCTGAACAAGG-3′ | |
|
| ||
| VEGFR-1 | Sense | 5′-TACCTCACCGTGCAAGGAA-3′ |
| Antisense | 5′-GAGTTAGAAGGAGCCAAAAGAGTG-3′ | |
|
| ||
| VEGFR-2 | Sense | 5′-CCCCAAATTCCATTATGACAA-3′ |
| Antisense | 5′-GCTTTTTCGCTTGCTGTTCT-3′ | |
|
| ||
| GAPDH | Sense | 5′-ACTCCCATTCTTCCACCTTTG-3′ |
| Antisense | 5′-TGTAGCCATATTCATTGTCATACC-3′ | |
VEGFA, vascular endothelial growth factor A; VEGFR-1, vascular endothelial growth factor receptor-1; VEGFR-2, vascular endothelial growth factor receptor-2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.