Figure 1.

Effect of Tt-ME on LPS-induced RAW264.7 and peritoneal macrophages. (a) Macrophages like RAW264.7 were pretreated with increasing concentrations of Tt-ME for 30 minutes before treatment with LPS for 24 h. The NO level in the solution was then determined. (b) RAW264.7 cells were treated with indicated concentrations of Tt-ME for 24 h after which viability of the Tt-ME-treated cells was then determined using an MTT assay. (c) Peritoneal macrophages were obtained from peritoneal exudates of thioglycollate-injected mice. NO levels in LPS-treated peritoneal macrophages in the presence or absence of Tt-ME and L-NAME were determined using the Griess assay. (d) Cell viability of peritoneal macrophages was determined in Tt-ME- and L-NAME-treated samples using an MTT assay. The results are representative of three independent experiments and are expressed as mean ± SD. ^∗p > 0.05 versus controls.