Table 1.
Semiquantitative PCR and real-time PCR primer sequences used in the study.
| Primer name | Direction | Sequence (5′ to 3′) |
|---|---|---|
| Semiquantitative PCR | ||
| iNOS | Forward | CCCTTCCGAAGTTTCTGGCAGCAG |
| Reverse | GGCTGTCAGAGCCTCGTGGCTTTGG | |
| IL-6 | Forward | CACTACATCCTGACCCACTT |
| Reverse | ATGCTCCTGCTTGAGTATGT | |
| TNF-α | Forward | TTGACCTCAGCGCTGAGTTG |
| Reverse | CCTGTAGCCCACGTCGTAGC | |
| GAPDH | Forward | CACTCACGGCAAATTCAACGGCA |
| Reverse | GACTCCACGACATACTCAGCAC | |
|
| ||
| Real-time PCR | ||
| iNOS | Forward | GGAGCCTTTAGACCTCAACAGA |
| Reverse | TGAACGAGGAGGGTGGTG | |
| COX-2 | Forward | CACTACATCCTGACCCACTT |
| Reverse | ATGCTCCTGCTTGAGTATGT | |
| TNF-α | Forward | GCCTCTTCTCATTCCTGCTTG |
| Reverse | CTGATGAGAGGGAGGCCATT | |
| IFN-β | Forward | AAGAGTTACACTGCCTTTGCCATC |
| Reverse | CACTGTCTGCTGGTGGAGTTCATC | |
| IFN-γ | Forward | GGGTTGTTGACCTCAAACTTGGCA |
| Reverse | CAGGCCATCAGCAACAACAT | |
| IL1-β | Forward | CAACCAACAAGTGATATTCTCCATG |
| Reverse | GATCCACACACTCCAGCTGCA | |
| IL-6 | Forward | CTAGGTTTGCCGAGTAGATCTC |
| Reverse | GACAAAGCCAGAGTCCTTCAGAGA | |
| iNOS | Forward | AACAATTCCTGGCGTTACCTT |
| Reverse | CTGCCGTACAACTCCAGTGA | |
| GAPDH | Forward | CAATGAATACGGCTACAGCAAC |
| Reverse | AGGGAGATGCTCAGTGTTGG | |