Table 1.

Primers and PCR conditions used for detection of virulence genes in E. coli isolated from Obushera.

Gene	Primers	PCR conditions	Base pairs	References
stx 1	5′CTTCGGTATCCTATTCCCGG3′ 3′GGATGCATCTCTGGTCATTG5′	30 cycles (94°C, 30 s; 56°C, 30 s; 72°C, 30 s)	448	[25]
stx 2	5′CCATGACAACGGACAGCAGTT3′ 3′CCTGTCAACTGAGCAGCACTTTG5′	30 cycles (94°C, 30 s; 56°C, 30 s; 72°C, 30 s)	779	[25]
eae	5′GTGGCGAATACTGGCGAGACT3′ 3′CCCCATTCTTTTTCACCGTCG5′	30 cycles (94°C, 30 s; 55°C, 30 s; 72°C, 1 min)	891	[26]