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. Author manuscript; available in PMC: 2020 Dec 24.
Published in final edited form as: Biochemistry. 2019 Dec 10;58(51):5102–5106. doi: 10.1021/acs.biochem.9b00891

Figure 2.

Figure 2.

Pyrvinium activates the CK1α splice variant CK1αS. (A) Schematic of CK1α splice variants. (B) Plasmids expressing HA-tagged CK1α splice variants were transfected into HEK293T cells for 48 h, followed by treatment with DMSO or 200 nM pyrvinium for 30 min. Cell lysates were resolved by SDS-PAGE and subjected to immunoblotting for the indicated proteins. Representative immunoblots are shown (n = 3). (C, D) Quantification (mean ± S.E.M., n = 3) of CK1α activity in response to each indicated splice variant from cells treated with a vehicle (DMSO) or pyrvinium is shown. CK1α activity indicates the level of P–S45 β-catenin normalized to that of total β-catenin and then normalized to that of HA-CK1α. An asterisk indicates statistical significance (Student’s t test, p ≤ 0.05), and NS indicates no statistical significance (Student’s t test, p > 0.05).