. Author manuscript; available in PMC: 2020 Dec 24.

Published in final edited form as: Biochemistry. 2019 Dec 10;58(51):5102–5106. doi: 10.1021/acs.biochem.9b00891

Table 1.

Summary of CK1αS Enzymatic Parameters

Enzymatic parameters	α-Caaein			ATP
Enzymatic parameters	DMSO	Pyrvinium	VU-WS211	DMSO	Pyrvinium	VU-WS211
V_max (pmol·min⁻¹)	9.39 ± 0.49	13.62 ± 0.51 ^*	9.91 ±0.22	11.35 ± 0.14 ^NS	12.87 ±0.25 ^*	11.47 ± 0.20
K_m (μM)	5.26 ± 0.84	6.51 ± 0.95	8.83 ± 1.18	14.02 ± 0.86	15.50 ± 0.18	14.12 ± 0.52
k_cat(min⁻¹)	4.69 ± 0.24	6.81 ± 0.26 ^*	4.95 ± 0.11	5.68 ± 0.07 ^NS	6.44 ±0.13 ^*	5.73 ± 0.10
k_cat/K_m (μM⁻¹·min⁻¹)	0.88 ± 0.12	1.15 ± 0.13 ^*	0.66 ± 0.18	0.38 ± 0.01	0.43 ± 0.01 ^*	0.40 ± 0.01

^*:

p value ≤ 0.05, compared to Group “α-Casein, DMSO”,

^NS:

p value >0.05, compared to Group “α-Casein, DMSO”,

^*:

p values ≤ 0.05, compared to Group “ATP, DMSO”, Student’s t-test.

Summarized enzymatic parameters of CK1αS (mean ± S.E.M.) were calculated from the mean ± SD (n = 2) of three independent biological replicates. Representative data of such experiments are shown in Figure 4. An asterisk indicates statistical significance compared to the relevant DMSO control (Student’s t test, p ≤ 0.05), and NS indicates no statistical significance (Student’s t test, p > 0.05).