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. 2019 Nov 28;48(1):332–348. doi: 10.1093/nar/gkz1095

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — S. meliloti YbeY co-ordinates zinc. (A) Different concentrations of the SmYbeY protein were prepared in activity buffer and the metal concentrations were calculated for magnesium (Dashed, filled circle), manganese (filled square), cobalt (filled triangle), nickel (inverted triangle) and zinc (open diamond) using ICP-MS. Unlike other metals, the measured concentration of Zn was proportional to the concentration of the protein used. (B) YbeY protein was exchanged from Buffer into metal-free water and analyzed using ICP-MS. Zinc was found to be stably associated with the SmYbeY protein. (C) SmYbeY was purified from cells supplemented with 0.1 mM Zn (SmYbeY^Zn) and the metals in the sample were analyzed using ICP-MS. By this method, the zinc occupancy of the protein was further enriched.