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. 2019 Nov 28;48(1):332–348. doi: 10.1093/nar/gkz1095

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — (A) SmYbeY complements the rRNA processing defect in EcΔybeY. Total RNA was extracted from the E. coli strain lacking YbeY (EcΔybeY) and carrying either the empty vector (pWSK29) or the plasmid expressing S. meliloti YbeY (pWSK-SmYbeY) and were subjected to electrophoresis in an Agarose/Synergel mix gel. The accumulation of the precursor 17S and the 16S* degradation product were reduced in the EcΔybeY strain expressing SmYbeY. (B) EcYbeY complements the rRNA processing defect in SmYbeY. Total RNA extracted from the S. meliloti strain lacking YbeY (SmΔybeY) and carrying either the empty vector (pWSK29) or the plasmid expressing E. coli YbeY (pWSK-EcYbeY) were subjected to electrophoresis similar to the A panel. The accumulation of the pre-16S rRNA product was reduced in the SmΔybeY strain expressing EcYbeY.