Table 3.

Prepared anti-ApoB-gapmers with their sequence and their melting temperature versus their complementary RNA strand. T_m is given as the average between the heating and the cooling curve

Entry	Denotation	Modification	Sequence	Tm (°C)
1	anti-ApoB-gapmer (control)	None	GmCattggtatTmCA	59.4
2	anti-ApoB-gapmer-5	3′S	GmCa(3′S)ttggtatTmCA	60.0
3	anti-ApoB-gapmer-6	3′S	GmCat(3′S)tggtatTmCA	62.5
4	anti-ApoB-gapmer-1	3′S	GmCatt(3′S)ggtatTmCA	61.0
5	anti-ApoB-gapmer-2	3′S	GmCattg(3′S)gtatTmCA	62.5
6	anti-ApoB-gapmer-7	3′S	GmCattgg(3′S)tatTmCA	62.5
7	anti-ApoB-gapmer-3	3′S	GmCattggt(3′S)atTmCA	62.5
8	anti-ApoB-gapmer-4	3′S	GmCattggta(3′S)tTmCA	61.9
9	anti-ApoB-gapmer-8	3′S	GmCattggtat(3′S)TmCA	63.5
10	anti-ApoB-gapmer-9	5′S	GmC(5′S)attggtatTmCA	56.4
11	anti-ApoB-gapmer-10	5′S	GmCa(5′S)ttggtatTmCA	58.5
12	anti-ApoB-gapmer-11	5′S	GmCat(5′S)tggtatTmCA	57.4
13	anti-ApoB-gapmer-12	5′S	GmCatt(5′S)ggtatTmCA	56.4
14	anti-ApoB-gapmer-13	5′S	GmCattg(5′S)gtatTmCA	59.0
15	anti-ApoB-gapmer-14	5′S	GmCattgg(5′S)tatTmCA	58.5
16	anti-ApoB-gapmer-15	5′S	GmCattggt(5′S)atTmCA	59.5
17	anti-ApoB-gapmer-16	5′S	GmCattggta(5′S)tTmCA	59.0

Positions of 3′S and 5′S linkages are indicated by (3′S) and (5′S) between bold nucleotides; A, G, ^mC, T represent LNA nucleotides; a, g, c, t represent DNA nucleotides; all other linkages were prepared as non-bridging phosphorothioates.