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Host portability of evolved aTF biosensors variants. (A) Schematic outline of biosensor prototyping on different chassis. (B) Dose–response curves for BenM variant TISNO-120 (BenM(TISNO120)) with evolved specificity for adipic acid biosensing versus no BenM expression (reporter-only) in E. coli. Data represents mean fluorescence intensities for mCherry/OD ± SD from three biological replicates. a.u. = arbitrary units.
