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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Kidney Int. 2019 Aug 13;97(1):119–129. doi: 10.1016/j.kint.2019.07.016

Figure 3. Twist1 in the distal nephron exacerbates kidney fibrosis induced by AA.

Figure 3.

(A) mRNA expression of Col-I, Pai-1, TGFβ1, and E-cadherin in the kidneys from WT and KKO mice at 5wks after AA injection (n≥8). (B) Total collagen content measured by Sirius red / fast green analysis of tissue sections from WT and KKO kidneys after AA exposure. (C) Representative sections from WT and KKO kidneys stained with Col-I at 5wks after AA injection. Scale bar =100 μm. (D) Blinded morphometric quantitation of Col-I (n≥8). (E) Western blot for FN, Col-I and α-SMA in whole kidney at 5wks after AA injection. (F) Semi-quantification of FN, Col-I and α-SMA from (E) (n≥8). Data represent the mean± SD (*p<0.05, **p<0.01, ***p<0.001).