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. 2019 Dec 14;10(1):158–167. doi: 10.1002/2211-5463.12766

Figure 3.

Figure 3

RPN2 overexpression promoted osteoblastic differentiation of hBMSCs. hBMSCs received transfection with the help of RPN2‐expressing vector, before osteoblastic differentiation stimulation. (A, B) qRT‐PCR (A) and WB (B) confirmed an up‐regulated RPN2 level at day 14. (C) Alizarin Red staining and von Kossa staining at the 14th day after stimulation. (D) Determination of ALP activity in every group at the 14th day after stimulation. (E) qRT‐PCR was used to examine the expression of OPN, RUNX2, OCN, ALP and BSP of every group at the 14th day after stimulation. These tests were repeated three times. Differences were assessed by two‐tailed Student's t‐test. Error bars represent SD. *P < 0.05 versus induced/negative control (NC) groups.