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. 2019 Dec 14;10(1):158–167. doi: 10.1002/2211-5463.12766

Figure 5.

Figure 5

RPN2 regulated the JAK1/STAT3 pathway. hBMSCs received transfection with the help of RPN2 siRNA or NC siRNA before induction of osteoblastic differentiation. (A) WB showed expression of JAK1 and STAT3, and phosphorylation of STAT3 after RPN2 silencing. (B) WB revealed the ubiquitination level of JAK1 after RPN2 silencing. (C) Immunofluorescence assay was used to detect the nuclear location of STAT3. Scale bar, 50 μm. These tests were repeated three times: *P < 0.05, **P < 0.01, ***P < 0.001 versus noninduced groups; # P < 0.05, ## P < 0.01, ### P < 0.001 versus induced/NCi group. DAPI, 4',6‐diamidine‐2'‐phenylindole dihydrochloride; IP, immunoprecipitation; NCi, negative control siRNA; Ub, ubiquitination.