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. 2019 Dec 14;10(1):158–167. doi: 10.1002/2211-5463.12766

Figure 6.

Figure 6

Assessment of the impact of JAK1 on osteoblastic differentiation modulated via RPN2. hBMSCs were cotransfected with RPN2 siRNA and/or JAK1 siRNA before stimulation of osteoblastic differentiation. (A–C) qRT‐PCR (A, B) and WB (C) were conducted to detect protein levels of RPN2 and JAK1. (D) ALP function was examined in every group during the osteoblastic differentiation. (E) qRT‐PCR was performed to examine the osteoblastic markers (OCN, OPN, RUNX2, BSP and ALP) of every group on day 14 after stimulation. These tests were repeated three times. Differences were assessed by ANOVA test. Error bars represent SD. *P < 0.05 versus induced/NCi group; ***P < 0.001; # P < 0.05 versus induced/RPN2i group. NCi, negative control siRNA.