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. 2019 Dec 10;10(1):18–27. doi: 10.1002/2211-5463.12732

Figure 4.

Figure 4

DANCR directly interacts with miR‐33b in PC cells. (A) Sequence alignment of miR‐33b with the putative binding sites within the wild‐type and mutant regions of DANCR. (B) Expression of miR‐33b was measured through qRT‐PCR in PC tissues and healthy adjacent tissues. Data are expressed as mean ± SD, Student's t‐test. (C) qRT‐PCR analysis was used to determine miR‐33b expression in AsPC‐1, PANC‐1, CFPAC‐1, SW1990, BxPC‐3, and HPDE6‐C7 cell lines. Data are expressed as mean ± SD, one‐way ANOVA. (D) miR‐33b expression was measured through qRT‐PCR in PANC‐1 and SW1990 cells transfected with shDANCR or shNC. Data are expressed as mean ± SD, Student's t‐test. (E) Relative luciferase activity of reporters containing DANCR‐Wt or DANCR‐Mt fragments in the indicated cells cotransfected with the indicated reporters and miR‐33b mimics or NC mimics. Data are expressed as mean ± SD, Student's t‐test. (F) The mRNA expression level of DANCR in 30 PC tissues was inversely related to miR‐33b expression. *P < 0.05.